Abstract:
Background: Polymorphism of genes that regulate fat metabolism in obese patients can be considered as of the predictors effectiveness of diet therapy. Aim: The study was to investigate the genetic predictors of standard low-calorie diet at different polymorphic variations ApoE and LPL genes in obese children.
Materials and methods: Our study involved 88 obese teenagers aged 15 to 19 years. Study of lipid metabolism includes the determination of total cholesterol, triglycerides, low-density lipoprotein, high-density lipoprotein determined by spectrophotometry and immuno turbidimetry. Analysis of polymorphisms of LPL gene Ser447Ter (c.1595C>G), Cys112Arg (c.388T>C) and Arg158Cys (c.526C>T) of ApoE gene was performed by polymerase chain reaction.
Results: The results show significant differences in the efficacy of diet, depending on the genotype. We found that obese teenagers have significantly higher carrier genotypes c.388 T/T + c.526 C/C (by 20% reduced total cholesterol, b y19% - triglycerides, by20 % - low density lipoprotein, by 16% - high density lipoprotein, and by 9% - glucose), c.388 T/T + c.526 C/T ApoE gene (by 24% reduced total cholesterol, by 20% - triglycerides, and by 2% increased level of highdensity lipoprotein) and C/C LPL gene (by 18% reduced total cholesterol concentration, by 17% - triglycerides, by 16% - high density lipoprotein, by 18% - low density lipoprotein, and by 9% - glucose).
Conclusions: Thus, ApoE and LPL genes alleles can be used as biomarkers of prognosis of efficiency of a standard low-calorie diet therapy in obese children.
